|
Variable /
Process |
Method /
Proceeding |
|
33P
uptake and part.
P |
|
|
Algal and
bacterial 13C |
Use of polar
lipid fatty acids as biomarker for algae and bacteria by adding
13C as a deliberate tracer |
|
Bacterial
Production (BPP), Bacterial Cell Numbers (free-living and attached
bacteria separated) |
|
|
Chlorophyll-a |
Fluorometry
of extracts |
|
Organic
matter degradation |
|
|
DOC (Dissolved
Organic Carbon) |
|
|
DON (Dissolved
Organic Nitrogen) |
|
|
DOP (Dissolved
Organic Phosphorus) |
|
|
E. huxleyi cell
cycle |
Flow Cytometry |
|
E. huxleyi
predation by protozoa |
Flow Cytometry
using fluorescently labelled E. huxleyi |
|
Gene expression involved in
carbon metabolism of Emiliania huxleyi |
|
|
Elemental
composition of bacteria, with the specific aim of looking at shifts in
Mg content |
X-ray
microanalysis (XRMA) |
|
Description of
population dynamics of viruses, bacteria, phytoplankton, (protozoa) |
|
|
Microzooplankton
grazing on E. huxleyi and partial dissolution of CaCO3 |
Serial dilution
technique |
|
Phytoplankton
pigments |
HPLC |
|
Phytoplankton
species composition |
Microscopy, Flow
Cytometry |
|
POC (Particulate
Organic Carbon) |
GC
CHN-analyzer |
|
PON (Particulate
Organic Nitrogen) |
GC
CHN-analyzer |
|
Quantification of
copepod egg production, hatching success and faecal pellet production |
Microscopy |
|
Quantification of
copepod grazing on E. huxleyi (and possibly other prey types) |
Quantitative PCR
on copepods and their faecal pellets |
|
Quantification of
mesozooplankton type and abundance |
Microscopy |
|
Quantification of
microzooplankton and copepod grazing on all prey types |
Combined bottle
incubation experiments analysed by microscopy, HPLC and Chl.a |
|
Single cell
measurements of protein and DNA content |
|
|
Sinking particles |
Sediment traps |
|
Sorting of E.
huxleyi to measure 33PO4-uptake |
Flow Cytometry |
|
Total primary
production, ( fractionated: some days) |
14C in-situ
incubation |
|
TPC |
CHN Analyzer |
|
Viral diversity |
DGGE and PFGE
(different gel systems in genome research), Flow Cytometry |
|
13C
- DIC |
MS |
|
Acetone, methanol,
DMS, isoprene, acetaldehyde etc. in the headspace of
the mesocosms |
Proton
Transfer Reaction Mass Spectrometry ( PTR-MS)
|
|
Continuous O2
monitoring |
Optodes |
|
DIC (Dissolved
Inorganic Carbon) |
Continuous Flow
Analysis and Coulometry |
|
DMS (aqueous),
DMSP, DMSP lyase activity |
GC |
|
Halocarbons
(including CHBr3, CH3I,...), hydrocarbons (including isoprene) and
organic sulphur compounds (including DMS)
in the headspace
of the mesocosm and in the seawater |
Use of
GC-MS (Gas-Chromatography coupled with Mass Spectrometer)
|
|
Mono- and
polysaccharides |
Colorimetrically with TPTZ |
|
Nutrients (NO2,
NO3, NH4, PO4, Si(OH)4) |
Wet chemistry |
|
Oxygen consumption
/ production |
O2
optode |
|
pCO2 in
water and headspace of the mesocosms |
Infra-red analyser |
|
TAlk (Total
Alkalinity) |
Gran electro titration |
|
TEP
(Transparent Exopolymer Particles) |
Colorimetrically,
microscopy |
|
Trace metal
speciation |
Voltammetry |
|
Salinity |
CTD |
|
Temperature |
CTD |